Construction of a reduction-responsive oligonucleotide via a post-modification approach utilizing 4-nitrophenyl diazomethane

Abstract Herein, we describe the construction of a reduction-responsive oligonucleotide by post-modification an with diazo compound bearing 4-nitrobenzyl group as cleavable moiety. High-performance liquid chromatography and mass spectrometry were used to reveal introduction 5?-phosphate oligonucleotide, subsequent reduction-triggered recovery original oligonucleotide. The protocol for preparation this is simple it will have various applications in fields chemical synthetic biology.